Data/hora: 10/06/2024 06:49:54

Provedor de dados: | 5
País: | France
Título: | Microplastics induce dose-specific transcriptomic disruptions in energy metabolism and immunity of the pearl oyster Pinctada margaritifera
Autores: | Gardon, Tony; Morvan, Lucie; Huvet, Arnaud; Quillien, Virgile; Soyez, Claude; Le Moullac, Gilles; Le Luyer, Jeremy.
Data: | 2020-11
Ano: | 2020
Palavras-chave: | Microbeads; Marine bivalve; Differentially expressed genes; Stress response; Energy-limited tolerance.
Resumo: | A combined approach integrating bioenergetics and major biological activities is essential to properly understand the impact of microplastics (MP) on marine organisms. Following experimental exposure of polystyrene microbeads (micro-PS of 6 and 10 μm) at 0.25, 2.5, and 25 μg L−1, which demonstrated a dose-dependent decrease of energy balance in the pearl oyster Pinctada margaritifera, a transcriptomic study was conducted on mantle tissue. Transcriptomic data helped us to decipher the molecular mechanisms involved in P. margaritifera responses to micro-PS and search more broadly for effects on energetically expensive maintenance functions. Genes related to the detoxification process were impacted by long-term micro-PS exposure through a decrease in antioxidant response functioning, most likely leading to oxidative stress and damage, especially at higher micro-PS doses. The immune response was also found to be dose-specific, with a stress-related activity stimulated by the lowest dose present after a 2-month exposure period. This stress response was not observed following exposure to higher doses, reflecting an energy-limited capacity of pearl oysters to cope with prolonged stress and a dramatic shift to adjust to pessimum conditions, mostly limited and hampered by a lowered energetic budget. This preliminary experiment lays the foundation for exploring pathways and gene expression in P. margaritifera, and marine mollusks in general, under MP exposure. We also propose a conceptual framework to properly assess realistic MP effects on organisms and population resilience in future investigations.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00640/75213/75351.pdf

https://archimer.ifremer.fr/doc/00640/75213/75352.docx

DOI:10.1016/j.envpol.2020.115180

https://archimer.ifremer.fr/doc/00640/75213/

Editor: | Elsevier BV
Formato: | application/pdf
Fonte: | Environmental Pollution (0269-7491) (Elsevier BV), 2020-11 , Vol. 266 , N. Part 3 , P. 115180 (9p.)

Fonte:

info:eu-repo/semantics/openAccess

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