Data/hora: 06/06/2024 14:27:37

Provedor de dados: | 5
País: | France
Título: | Diversity of enterovirus sequences detected in oysters by RT-heminested PCR
Autores: | Dubois, Eric; Merle, Ghislaine; Roquier, Catherine; Trompette, Aurélien; Le Guyader, Soizick; Cruciere, Catherine; Chomel, Jean-jacques.
Data: | 2004-04
Ano: | 2004
Palavras-chave: | Sequence analyze; RT PCR; Cell culture; Shellfish; Enterovirus.
Resumo: | Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the different sites were different but similarities were observed between sequences detected in shellfish from two sites at different dates. Sequences were also compared to sequences of human, bovine and porcine enteroviruses. Both human and animal origins of enterovirus contamination of shellfish seemed likely.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2004/publication-763.pdf

DOI:10.1016/j.ijfoodmicro.2003.06.001

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/763/
Formato: | application/pdf
Fonte: | International Journal of Food Microbiology (0168-1605) (Elsevier), 2004-04 , Vol. 92 , N. 1 , P. 35-43
Fonte: | 2004 Elsevier B.V. All rights reserved