Data/hora: 14/06/2024 04:20:17

Provedor de dados: | 5
País: | France
Título: | Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions
Autores: | Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Data: | 2018-08
Ano: | 2018
Resumo: | Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. This obligatory intracellular parasite persists and multiplies into hemocytes. Previous in vitro experiments showed that apoptosis is activated in hemocytes between 1 h and 4 h of contact with the parasite. The flat oyster uses the apoptosis pathway to defend against B. ostreae. However, the parasite might be also able to modulate this response in order to survive in its host. In order to investigate this hypothesis the apoptotic response of the host was evaluated using flow cytometry, transmission electron microscopy and by measuring the response of genes involved in the apoptotic pathway after 4 h. In parallel, the parasite response was investigated by measuring the expression of B. ostreae genes involved in different biological functions including cell cycle and cell death. Obtained results allow describing molecular apoptotic pathways in O. edulis and confirm that apoptosis is early activated in hemocytes after a contact with B. ostreae. Interestingly, at cellular and molecular levels this process appeared downregulated after 44 h of contact. Concurrently, parasite gene expression appeared reduced suggesting that the parasite could inhibit its own metabolism to escape the immune response.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00454/56540/58246.pdf

http://archimer.ifremer.fr/doc/00454/56540/58247.xlsx

http://archimer.ifremer.fr/doc/00454/56540/58248.pdf

DOI:10.1038/s41598-018-29776-x

http://archimer.ifremer.fr/doc/00454/56540/

Editor: | Nature Publishing Group
Formato: | application/pdf
Fonte: | Scientific Reports (2045-2322) (Nature Publishing Group), 2018-08 , Vol. 8 , N. 1 , P. 12494 (12p.)

Fonte:

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