Data/hora: 16/06/2024 11:17:58

Provedor de dados: | 5
País: | France
Título: | Polysaccharide labelling: impact on structural and biological properties
Autores: | Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel.
Data: | 2002-11
Ano: | 2002
Palavras-chave: | Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide.
Resumo: | Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2002/publication-2113.pdf

DOI:10.1016/S0144-8617(02)00030-9

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/2113/
Formato: | application/pdf
Fonte: | Carbohydrate Polymers (0144-8617) (Elsevier), 2002-11 , Vol. 50 , N. 3 , P. 273-278
Fonte: | Elsevier