Data/hora: 11/06/2024 04:00:43

Provedor de dados: 	5	
País: 	France	
Título: 	Response of Listeria monocytogenes to liquid smoke	
Autores: 	Guilbaud, Morgan; Chafsey, Ingrid; Pilet, Marie-france; Leroi, Francoise; Prevost, Herve; Hebraud, Michel; Dousset, Xavier.	
Data: 	2008-06	
Ano: 	2008	
Palavras-chave: 	Survival; Stress; Proteome analysis; Listeria monocytogenes; Liquid smoke; Haemolysis.	
Resumo: 	Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes.   Methods and Results: Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 mu g ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 mu g ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 mu g ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly gene expression, showing a potential inhibition of protein activity or stability.   Conclusions: The presence of liquid smoke in a rich medium strongly affected growth and survival of L. monocytogenes. Brief smoke stress affected the metabolic pathways and inhibited the haemolytic activity of L. monocytogenes.   Significance and Impact of the Study: This study is a first step in the investigation of the influence of a smoked product on L. monocytogenes strains.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2008/publication-4522.pdf

DOI:10.1111/j.1365-2672.2008.03731.x	
Editor: 	Blackwell science	
Relação: 	http://archimer.ifremer.fr/doc/00000/4522/	
Formato: 	application/pdf	
Fonte: 	Journal of Applied Microbiology (1364-5072) (Blackwell science), 2008-06 , Vol. 104 , N. 6 , P. 1744-1753	
Fonte: 	2008 Wiley Blackwell Publishing, Inc. 2008 The Society for Applied Microbiology