Data/hora: 10/06/2024 14:09:53

Provedor de dados: | 5
País: | France
Título: | Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia)
Autores: | Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis.
Data: | 1991-08
Ano: | 1991
Palavras-chave: | Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae.
Resumo: | The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These monoclonal antibodies should prove to be of great value as diagnostic and research tools.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/1991/publication-2756.pdf
Editor: | Inter-Research
Relação: | http://archimer.ifremer.fr/doc/00000/2756/
Formato: | application/pdf
Fonte: | Diseases of aquatic organisms (Inter-Research), 1991-08 , Vol. 11 , P. 135-142
Fonte: | Inter-Research