Data/hora: 02/06/2024 15:41:20

Provedor de dados: 	5	
País: 	France	
Título: 	Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia)	
Autores: 	Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis.	
Data: 	1991-08	
Ano: 	1991	
Palavras-chave: 	Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae.	
Resumo: 	The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These monoclonal antibodies should prove to be of great value as diagnostic and research tools.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/1991/publication-2756.pdf	
Editor: 	Inter-Research	
Relação: 	http://archimer.ifremer.fr/doc/00000/2756/	
Formato: 	application/pdf	
Fonte: 	Diseases of aquatic organisms (Inter-Research), 1991-08 , Vol. 11 , P. 135-142	
Fonte: 	Inter-Research