Data/hora: 15/06/2024 10:19:23

Provedor de dados: 	5	
País: 	France	
Título: 	The toxicity of benzo[a]pyrene on sole (Solea solea) hepatocytes: assessment of genotoxic and enzymatic effects	
Autores: 	Wessel, Nathalie; Menard, Dominique; Pichavant-rafini, K.; Ollivier, H.; Le Goff, Jean-michel; Burgeot, Thierry; Akcha, Farida.	
Data: 	2010	
Ano: 	2010	
Palavras-chave: 	Genotoxicity; Sole; BaP; DNA strand breaks; EROD; DNA adducts.	
Resumo: 	The benzo[a]pyrene is a polycyclic aromatic hydrocarbon known to be genotoxic, mutagenic and carcinogenic in higher vertebrates. The aim of this study was to evaluate in vitro the enzymatic and genotoxic effects of BaP in a benthic fish species, Solea solea. Sole hepatocytes were exposed to BaP in order to measure the modulation of ethoxyresorufin-o-deethylase (EROD) activity and the DNA strand breaks induced by BaP metabolism. Exposures were performed in both culture flasks and microplate wells in order to check for the possible miniaturization of the exposure system. Moreover, sole liver microsomes were exposed to BaP in the presence of standard DNA in order to assess the potential formation of DNA adducts in sole. The results demonstrated the ability of sole hepatic enzymes to metabolize BaP into reactive species responsible for bulky DNA adducts and DNA strand breakage, whatever the tested exposure concentration and the mode of exposure.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00018/12968/9944.pdf	
Editor: 	Taylor &amp; Francis Ltd	
Relação: 	http://archimer.ifremer.fr/doc/00018/12968/	
Formato: 	application/pdf	
Fonte: 	Polycyclic Aromatic Compounds (1040-6638) (Taylor &amp; Francis Ltd), 2010 , Vol. 30 , N. 5 , P. 346-354	
Fonte: 	2010 Taylor &amp; Francis