Data/hora: 02/06/2024 15:34:51

Provedor de dados: | 5
País: | France
Título: | Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
Autores: | Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M.
Data: | 2007-09
Ano: | 2007
Palavras-chave: | Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding.
Resumo: | This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-3034.pdf

DOI:10.1111/j.1471-8286.2007.01863.x

Editor: | Blackwell science
Relação: | http://archimer.ifremer.fr/doc/00000/3034/
Formato: | application/pdf
Fonte: | Molecular Ecology Notes (1471-8278) (Blackwell science), 2007-09 , Vol. 7 , N. 5 , P. 730-734
Fonte: | 2007 Blackwell