Data/hora: 02/06/2024 05:03:25

Provedor de dados: | 52
País: | Brazil
Título: | Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles
Autores: | Leitão,Cintia Camurça Fernandes; Costa,José Jackson do Nascimento; Brito,Ivina Rocha; Magalhães-Padilha,Déborah de Melo; Almeida,Anderson Pinto; Figueiredo,José Ricardo de; van den Hurk,Robert; Silva,José Roberto Viana.
Data: | 2014-04-01
Ano: | 2014
Palavras-chave: | BMPs; Follicles; GDF-9; In vitro; MRNA.
Resumo: | The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for GDF-9, FSH-R and BMPs -2, -4, -6, -7 and -15 in cultured follicles were quantified by real time PCR. The results showed that a significant increase of follicle diameter after six days when compared to day 0, but the presence of GDF-9 and FSH did not influence the follicular growth in comparison with those cultured in MEM. Real time PCR showed that GDF-9 down-regulated the levels of mRNA for BMPs -2 and -15, while FSH either alone or in combination with GDF-9 did not affect the expression of GDF-9, FSH-R and BMPs. In conclusion, GDF-9 reduced the expression of BMP-2 and -15 in caprine preantral follicles after their culture, but FSH either alone or in association with GDF-9 did not control the expression of GDF-9, FSH-R and BMPs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200007
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132014000200007
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.57 n.2 2014
Fonte: | info:eu-repo/semantics/openAccess