Data/hora: 02/06/2024 13:46:06

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Isolation of natural inhibitors of papain obtained from Carica papaya latex	
Autores: 	Monti,Rubens; Contiero,Jonas; Goulart,Antonio José.	
Data: 	2004-09-01	
Ano: 	2004	
Palavras-chave: 	Esterase activity; Natural papain inhibitor; Protease inhibitor.	
Resumo: 	Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132004000500010	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.47 n.5 2004	
Fonte: 	info:eu-repo/semantics/openAccess