Data/hora: 09/06/2024 02:15:33

Provedor de dados: | 52
País: | Brazil
Título: | Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2
Autores: | Ferreira,Graziela Batista; Evangelista,Alex Ferreira; Severo Junior,João Baptista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile; Jordão,Elizabete.
Data: | 2007-05-01
Ano: | 2007
Palavras-chave: | Partitioning; Optimization; Aqueous two-phase systems; Maize malt; PEG 6000; CaCl2.
Resumo: | This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable for continuous aqueous two-phase purification due to the stability of proteins (e.g. and -amylases) and could increase their content into middle.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300020
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132007000300020
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.50 n.3 2007
Fonte: | info:eu-repo/semantics/openAccess