Data/hora: 08/06/2024 16:24:05

Provedor de dados: | 52
País: | Brazil
Título: | Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
Autores: | Wang,Yong; Huang,Wenhua; Peng,Daizhi; Hu,Yuanbin.
Data: | 2008-08-01
Ano: | 2008
Palavras-chave: | Nuclear transcription factor; Electrophoretic mobility shift assay; Methodology; Modification.
Resumo: | In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000400025
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess