Data/hora: 02/06/2024 14:57:31

Provedor de dados: | 52
País: | Brazil
Título: | Comparison of different methodologies for DNA extraction from Aegla longirostri
Autores: | Bitencourt,João Vitor Trindade; Roratto,Paula Angélica; Bartholomei-Santos,Marlise Ladvocat; Santos,Sandro.
Data: | 2007-11-01
Ano: | 2007
Palavras-chave: | Anomura; Aeglidae; Freshwater crab; DNA extraction.
Resumo: | The aim of this study was to compare some DNA extraction methodologies for Aegla longirostri. The protocols were based on the traditional phenol-chloroform DNA extraction methodology and using a commercial kit for DNA extraction. They differed in tissues used, the addition - or not - of beta-mercaptoethanol to the lysis buffer, times and methods for the animal's conservation (frozen, in ethanol or fresh). Individuals stored at -20°C for a long time supplied lower molecular weight DNA than those stored for a short time. The best yield for the specimens preserved in ethanol was obtained for 15 days storage in 95% ethanol. The kit resulted in a low quantity of high molecular weight DNA. The best protocol for DNA extraction from Aeglidae, and probably for other crustaceans should, therefore, utilize fresh specimens, with addition of beta-mercaptoethanol to the lysis buffer.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132007000700010
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.50 n.6 2007
Fonte: | info:eu-repo/semantics/openAccess