Data/hora: 05/06/2024 02:20:47

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates	
Autores: 	Oliveira,Flávia Márcia; Ruiz-de-Souza,Valéria; Campana-Pereira,Maria Aparecida; Gontijo,Cristiano Machado.	
Data: 	2013-12-01	
Ano: 	2013	
Palavras-chave: 	Ovalbumin; Palmitic Acid; Cellular Immunity; CD86; CD80; CD11b.	
Resumo: 	One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100ml of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro stimulation with palmitoyl-Ova; additionally, both Ova and palmitoyl-Ova induced the basal expression of CD11b. These findings could be related with the strong T-cell proliferative response induced by palmitoyl-Ova.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600011	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132013005000003	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.56 n.6 2013	
Fonte: 	info:eu-repo/semantics/openAccess