Data/hora: 01/06/2024 17:30:10

Provedor de dados: | 52
País: | Brazil
Título: | Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp
Autores: | Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
Data: | 2009-04-01
Ano: | 2009
Palavras-chave: | Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product.
Resumo: | The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and concentrated in 10 fold the bromelain extract. SDS-PAGE electrophoresis showed that the ultra-filtrated had high purity and the bromelain from A. comosus pulp had a molecular weight of 24.5 kDa.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000200024
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess