Data/hora: 01/06/2024 16:49:23

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp	
Autores: 	Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.	
Data: 	2009-04-01	
Ano: 	2009	
Palavras-chave: 	Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product.	
Resumo: 	The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and concentrated in 10 fold the bromelain extract. SDS-PAGE electrophoresis showed that the ultra-filtrated had high purity and the bromelain from A. comosus pulp had a molecular weight of 24.5 kDa.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132009000200024	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.52 n.2 2009	
Fonte: 	info:eu-repo/semantics/openAccess