Data/hora: 16/06/2024 08:43:13

Provedor de dados: | 52
País: | Brazil
Título: | Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431
Autores: | Mohansrinivasan,V.; Devi C.,Subathra; Deori,Meenakshi; Biswas,Ananya; Naine S,Jemimah.
Data: | 2015-08-01
Ano: | 2015
Palavras-chave: | GSE (Grape seed extract); Phytochemical; FTIR; GCMS; Skin cancer cell line; Anticancer agents.
Resumo: | In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate, ethyl-9- cis -11- trans octadecadienoate and (R)-(-)-14,-methyl-8-Hexadecyn-1-ol in petroleum ether extract. Cytotoxic activity of the GSE was observed against skin cancer cell lines A4321 using 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide) MTT assay. The IC50 value of the GSE against A431 skin cancer cell line was 480 µg/mL. This is first such report against A4321 cell lines. The study gives the overall perception about importance of GSE in medicine and nutraceuticals purposes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400540
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/s1516-8913201500076
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess