Data/hora: 07/06/2024 07:35:05

Provedor de dados: | 52
País: | Brazil
Título: | Induced immune response of Escherichia coli BL21 expressing recombinant MSP1a and MSP1b proteins of Anaplasma marginale
Autores: | Tamekuni,Katia; Vidotto,Marilda Carlos; Felix,Samuel Rodrigues; Igarashi,Michelle; Garcia,João Luis; Coelho,Adriana Letícia; Gasparini,Marcela Ribeiro; Marana,Elizabete Regina Marangoni; Silva,Sérgio Silva da; Vidotto,Odilon.
Data: | 2009-11-01
Ano: | 2009
Palavras-chave: | Anaplasma marginale; MSP1a; MSp1b; Vaccine; Mice; Cattle.
Resumo: | This work aims to evaluate the potential of immunization with E. coli BL21 expressing the recombinant rMSP1a and rMSP1b proteins of Anaplasma marginale. E. coli BL21 was transformed with recombinant plasmids pET102/msp1α and pET101/msp1β, and rMSP1a and rMSP1b were expressed after induction by IPTG. BALB/c mice were vaccinated with formolized BL21/rMSP1a and BL21/rMSP1b, and the production in mice sera of whole IgG was determined by ELISA. The mice immunized with BL21/rMSP1a showed a better humoral response for whole IgG when compared to the mice immunized with BL21/rMSP1b; these mice exhibited a small response after the second vaccination. Sera of mice immunized with BL21/rMSP1a reacted via western blot with BL21 and rMSP1a, with molecular masses varying from 70 to 105 kDa. Sera of mice immunized with BL21/rMSP1b reacted with BL21 and rMSP1b with a molecular mass of 100 kDa. These results demonstrate that BL21 containing rMSP1a and rMSP1b in the outer membrane were able to produce an immune response in mice, reinforcing its use in vaccine models against bovine anaplasmosis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700016
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000700016
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.spe 2009
Fonte: | info:eu-repo/semantics/openAccess