Data/hora: 01/06/2024 06:27:13

Provedor de dados: | 52
País: | Brazil
Título: | Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate
Autores: | Braunig,Patricia; Rosa,Rafael Diego da; Seibert,Caroline Heidrich; Borsa,Mariana; Stoco,Patricia Hermes; Grisard,Edmundo Carlos; Pinto,Aguinaldo Roberto.
Data: | 2011-04-01
Ano: | 2011
Palavras-chave: | WSSV; VP28; Recombinant protein; Penaeid shrimp.
Resumo: | In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132011000200023
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.54 n.2 2011
Fonte: | info:eu-repo/semantics/openAccess