Data/hora: 01/06/2024 10:00:50

Provedor de dados: | 52
País: | Brazil
Título: | The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS
Autores: | Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
Data: | 2011-02-01
Ano: | 2011
Palavras-chave: | Bromelain; Aqueous two-phase systems; Partitioning; Characterization; Pineapple.
Resumo: | In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40ºC.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100017
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132011000100017
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.54 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess