Data/hora: 06/06/2024 03:37:36

Provedor de dados: | 52
País: | Brazil
Título: | Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves
Autores: | Stipp,Danilo Tancler; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Bodnar,Lívia; Alfieri,Amauri Alcindo.
Data: | 2009-11-01
Ano: | 2009
Palavras-chave: | Cattle; Diarrhea; Bovine coronavirus; Cell culture; Molecular analyses.
Resumo: | Bovine coronavirus (BCoV) may cause acute diarrhea in newborn calves, leading to significant economic losses for cattle farmers. There are several diagnostic techniques used to detect BCoV in calf fecal samples, but virus isolation still has advantages for antigenic and genomic characterization. This study describes the isolation in HRT-18 cells and molecular characterization of Brazilian BCoV wild-type strains. Three fecal samples from diarrheic 30 day-old calves were inoculated in HRT-18 cell monolayers, which were then evaluated for HA titers and tested using semi-nested PCR followed by RFLP and sequencing. Two samples were successfully isolated and presented HA titers of 16 and 32 units per 25 mL. The results were confirmed using semi-nested PCR and RFLP. Molecular analyses identified a cell culture-adapted strain and a wild-type strain that were genetically similar (99%) to each other, but more distinct than BCoV strains circulating in other countries, even in the conserved N gene.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700007
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000700007
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.spe 2009
Fonte: | info:eu-repo/semantics/openAccess