Data/hora: 01/06/2024 03:31:22

Provedor de dados: | 52
País: | Brazil
Título: | Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer
Autores: | Oda,Sandra Helena Inoue; Nepomuceno,Alexandre Lima; Ledur,Mônica Corrêa; Oliveira,Maria Cristina Neves de; Marin,Silvana Regina Rockenbach; Ida,Elza Iouko; Shimokomaki,Massami.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | Malignant hyperthermia; Poultry; Gene expression; Real time PCR.
Resumo: | Total RNA isolated from Pectoralis major muscle from PSE (L*24h&gt;53.0, pH&lt;5.8) and non-PSE (44&lt;"L*24h&gt;"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p&lt;0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p&gt;0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca2+ availability at the cell milieu.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600024
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000600024
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.6 2009
Fonte: | info:eu-repo/semantics/openAccess