Data/hora: 01/06/2024 00:49:14

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer	
Autores: 	Oda,Sandra Helena Inoue; Nepomuceno,Alexandre Lima; Ledur,Mônica Corrêa; Oliveira,Maria Cristina Neves de; Marin,Silvana Regina Rockenbach; Ida,Elza Iouko; Shimokomaki,Massami.	
Data: 	2009-12-01	
Ano: 	2009	
Palavras-chave: 	Malignant hyperthermia; Poultry; Gene expression; Real time PCR.	
Resumo: 	Total RNA isolated from Pectoralis major muscle from PSE (L*24h&gt;53.0, pH&lt;5.8) and non-PSE (44&lt;"L*24h&gt;"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p&lt;0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p&gt;0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca2+ availability at the cell milieu.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600024	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132009000600024	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.52 n.6 2009	
Fonte: 	info:eu-repo/semantics/openAccess