Data/hora: 07/06/2024 04:56:41

Provedor de dados: | 52
País: | Brazil
Título: | Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli
Autores: | Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species.
Resumo: | Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play a role in DNA repair. On the whole, data has shown that the alkaline gel electrophoresis assay could be used both for studying DNA strand breaks induction and for associated repair mechanisms.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000700020
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.spe 2008
Fonte: | info:eu-repo/semantics/openAccess