Data/hora: 08/06/2024 10:31:20

Provedor de dados: | 52
País: | Brazil
Título: | Batch and continuous extraction of bromelain enzyme by reversed micelles
Autores: | Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
Data: | 2009-10-01
Ano: | 2009
Palavras-chave: | Reversed micelles; Liquid-liquid extraction; Bromelain; Pineapple; Optimization; Surface response.
Resumo: | The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000500021
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.5 2009
Fonte: | info:eu-repo/semantics/openAccess