Data/hora: 02/06/2024 15:46:30

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Cloning of high molecular weight gluten subunit promoter and study on its function in wheat	
Autores: 	Weibo,Jin; Jin,Liu; Fangli,Wu; Aiguang,Guo.	
Data: 	2009-04-01	
Ano: 	2009	
Palavras-chave: 	Triticum aestivum; Glutenin; Promoter; Endosperm-specific; Transient expression.	
Resumo: 	The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132009000200002	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.52 n.2 2009	
Fonte: 	info:eu-repo/semantics/openAccess