Data/hora: 05/06/2024 06:29:47

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG	
Autores: 	Penha,Tânia Regina; Krüger,Ernesto Renato; Thomaz-Soccol,Vanete; Agottani,Jorge Victor Bacila; Itano,Flávio Hiroshi; Troiano,Ludmilla Della Coletta; Brodzinski,Josiane.	
Data: 	2010-02-01	
Ano: 	2010	
Palavras-chave: 	Monoclonal antibodies; Bovine immunoglobulin G; Fragment Fc.	
Resumo: 	The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132010000100014	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.53 n.1 2010	
Fonte: 	info:eu-repo/semantics/openAccess