Data/hora: 16/06/2024 09:43:26

Provedor de dados: | 52
País: | Brazil
Título: | Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides
Autores: | Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros.
Data: | 2011-08-01
Ano: | 2011
Palavras-chave: | Marine algae; Sulfated macromolecules; Chemical analysis; APTT test.
Resumo: | The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg-1), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132011000400007
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.54 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess