Data/hora: 01/06/2024 00:55:42

Provedor de dados: | 52
País: | Brazil
Título: | Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
Autores: | Mariano,André Bellin; Kovalhuk,Leonardo; Valente,Caroline; Maurer-Menestrina,Juliana; Pereira-Netto,Adaucto Bellarmino; Guerra,Miguel Pedro; Carnieri,Eva Gunilla Skare.
Data: | 2004-11-01
Ano: | 2004
Palavras-chave: | Araucaria angustifolia; Callus; Plant mitochondria; Respiratory chain.
Resumo: | A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132004000600006
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.47 n.6 2004
Fonte: | info:eu-repo/semantics/openAccess