Data/hora: 01/06/2024 02:27:10

Provedor de dados: | 52
País: | Brazil
Título: | Production of specific polyclonal antibodies anti-human factor IX
Autores: | Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
Data: | 2006-07-01
Ano: | 2006
Palavras-chave: | Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Resumo: | In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132006000500010
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.49 n.4 2006
Fonte: | info:eu-repo/semantics/openAccess