Data/hora: 06/06/2024 18:13:31

Provedor de dados: | 52
País: | Brazil
Título: | Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
Autores: | Bezerra,Raquel Pedrosa; Borba,Fernanda Katharine Sousa Lins; Moreira,Keila Aparecida; Lima-Filho,José Luis; Porto,Ana Lúcia Figueiredo; Chaves,Adilson Castro.
Data: | 2006-07-01
Ano: | 2006
Palavras-chave: | Amylases; Aqueous two-phase systems; Bacillus subtilis; Partition; Poly (ethylene glycol).
Resumo: | Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132006000500003
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.49 n.4 2006
Fonte: | info:eu-repo/semantics/openAccess