Data/hora: 08/06/2024 12:35:45

Provedor de dados: | 52
País: | Brazil
Título: | Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation
Autores: | Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
Data: | 2012-10-01
Ano: | 2012
Palavras-chave: | Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Resumo: | This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola's.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132012000500002
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.55 n.5 2012
Fonte: | info:eu-repo/semantics/openAccess