Data/hora: 06/06/2024 06:34:42

Provedor de dados: 	52	
País: 	Brazil	
Título: 	A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery	
Autores: 	Severo Jr.,João Batista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.	
Data: 	2009-04-01	
Ano: 	2009	
Palavras-chave: 	BSA; Protein adsorption; Breakthrough curves; Amberlite IRA 410 ion-exchange resin; Distribution residence time study; Richardson-Zack coefficient.	
Resumo: 	In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200021	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132009000200021	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.52 n.2 2009	
Fonte: 	info:eu-repo/semantics/openAccess