Data/hora: 05/06/2024 16:39:30

Provedor de dados: | 52
País: | Brazil
Título: | Radiolabeling and biodistribution of monoclonal antibody (MAb) anti-CD20 with iodine-131
Autores: | Akanji,Akinkunmi Ganiyu; Muramoto,Emiko; Caldeira Filho,José de Souza; Couto,Renata Martinussi; Araújo,Elaine Bortoletti de.
Data: | 2005-10-01
Ano: | 2005
Palavras-chave: | Monoclonal antibody; Radioioactivity; Anti-CD20; Radioimmunotherapy.
Resumo: | Radioactive isotopes of iodine, mainly iodine-131 have been broadly used in many monoclonal antibody radioiodination settings, employing different methods. In this study, using a Chloramine-T procedure, the influence of incubation time, CT mass, and Ab/activity ratio on the radiochemical yield of the anti-CD20 antibody labeled with iodine-131 was observed. Radiochemical yield was &gt; 97 %, employing different Chloramine-T masses, independently of incubation time. Radiochemical purity was above 99 % after purification of the labeled compound. The relationship between Ab mass and radioactivity was tested and no difference was observed when 90.6 MBq (2.45mCi) of activity was incorporated in the Ab-mass range studied. Biodistribution study in normal Swiss mice showed higher uptake by the liver and intestines. Low thyroid uptake indicated a suitable in vivo stability. Slight blood uptake was considered a result of circulating radioactivity in normal organs and tissues. A favorable biological distribution of 131I-anti-CD20 suggests this radiopharmaceutical may be effectively used in the therapy of Non Hodgkin Lymphoma.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700010
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132005000700010
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.48 n.spe2 2005
Fonte: | info:eu-repo/semantics/openAccess