Data/hora: 06/06/2024 13:39:42

Provedor de dados: | 52
País: | Brazil
Título: | Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption
Autores: | Sugahara,Vanessa Hitomi; Varéa,Geni da Silva.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Immobilization; Lipase; Beauveria bassiana; Silica gel.
Resumo: | Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no activity for esterification reaction in n-heptane. The treatment of ILS with 0.8 M NaCl prevented lipase desorption while Triton X-100 (0.1%) resulted the enzyme leakage. The ILS was reused for four times for esterification reaction with 80.8% of initial activity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-8913201401358
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.57 n.6 2014
Fonte: | info:eu-repo/semantics/openAccess