Data/hora: 14/06/2024 03:10:58

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant	
Autores: 	Sharmin,Shamima Akhtar; Alam,Md. Jahangir; Sheikh,Md. Mominul Islam; Sarker,Kanak Kanti; Khalekuzzaman,Muhammad; Haque,Md. Anwarul; Alam,Mohammad Firoz; Alam,Iftekhar.	
Data: 	2014-06-01	
Ano: 	2014	
Palavras-chave: 	In vitro; Medicinal plant; Micropropagation; Somatic embryo; Threatened; Wedelia calendulacea.	
Resumo: 	In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W.calendulacea. This result could facilitate genetic transformation of this important medicinal plant.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-8913201401840	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.57 n.3 2014	
Fonte: 	info:eu-repo/semantics/openAccess