Data/hora: 05/06/2024 05:40:07

Provedor de dados: | 52
País: | Brazil
Título: | Caffeine formation by suspension cultures of Coffea dewevrei
Autores: | Sartor,Rosana Mary; Mazzafera,Paulo.
Data: | 2000-01-01
Ano: | 2000
Palavras-chave: | Coffee; Caffeine; Cell suspension culture; Methylxanthines.
Resumo: | The low caffeine content in leaves of C. dewevrei (~ 0.5 mg/g) is due to a low biosynthesis associated with a fast degradation. On the other hand, high biosynthesis and low degradation confer a higher content (~ 8 mg/g) in leaves of C. arabica. In this work it was observed that cell cultures of C. dewevrei recovered the ability to synthesize caffeine almost in similar levels of C. arabica cultures. Tracer experiments with labelled carbon dioxide showed a significant accumulation of radioactivity in caffeine and metabolites, indicating an active biosynthesis. When the cultures were fed with labelled caffeine most of the radioactivity was recovered in caffeine, indicating that although active, degradation was not so efficient as in leaves, and therefore, contributing for the alkaloid accumulation in the cell cultures.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100009
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132000000100009
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.43 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess