Data/hora: 16/06/2024 11:03:02

Provedor de dados: | 52
País: | Brazil
Título: | Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
Autores: | Khan,Abdul Basit; Khattak,Mehmood Iqbal; Tarar,Omer Mukhtar; Habib,Farzana; Jamil,Khalid; Yasmin,Arfa; Parvez,Shoukat.
Data: | 2013-08-01
Ano: | 2013
Palavras-chave: | Polyhydroxyalkanoates; Biodegradable polymers; PHA synthase gene; FTIR.
Resumo: | In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132013000400015
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.56 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess