Data/hora: 06/06/2024 10:18:09

Provedor de dados: | 52
País: | Brazil
Título: | Characterization of Antimycins – Producing Streptomycete Strain VY46 Isolated from Slovak Soil
Autores: | Charousová,Ivana; Steinmetz,Heinrich; Medo,Juraj; Javoreková,Sona; Wink,Joachim.
Data: | 2016-01-01
Ano: | 2016
Palavras-chave: | Streptomycetes; Anticandidal activity; Metabolite production; Antimycins antibiotics.
Resumo: | ABSTRACT The strain no. VY46 was isolated from agricultural soil of Slovak republic and tested for potential antimicrobial activity against various human pathogens. On the basis of results, strain VY46 significantly inhibited growth of yeast Candida albicans and therefore was used for further characterization. In order to explore the potential bioactivities, extract of the fermented broth culture was prepared with organic solvent extraction method. The ethylacetate extract was subjected to HPLC fractionation against Candida albicans and followed by LC/MS analysis for potential production of anticandidal substances. The analysis resulted in the identification of two antimycins antibiotics, which may be responsible for important anticandidal activity of the strain. On the basis of liquid chromatography and mass spectrometry the antibiotics were identified as Urauchimycin A and Kitamycin A. According tothe results from cultural, morphological, physiological, biochemical and 16S rRNA gene sequence methods, the strain was identified as Streptomyces albidoflavus. In addition, neighbor-joining phylogenetic tree confirmed the relationships of this strain to other members of Streptomyces genera.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100349
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/1678-4324-2016160274
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.59 2016
Fonte: | info:eu-repo/semantics/openAccess