Data/hora: 06/06/2024 03:23:19

Provedor de dados: | 52
País: | Brazil
Título: | Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Autores: | Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Molecular identification; Real-time PCR; Tospovirus vector.
Resumo: | ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/1678-4324-2017160425
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.60 2017
Fonte: | info:eu-repo/semantics/openAccess