Data/hora: 08/06/2024 16:40:03

Provedor de dados: | 52
País: | Brazil
Título: | Effect of proteins from the red seaweed Hypnea musciformis (Wulfen) Lamouroux on the growth of human pathogen yeasts
Autores: | Cordeiro,Rossana Aguiar; Gomes,Valdirene Moreira; Carvalho,Ana Fontenele Urano; Melo,Vânia Maria Maciel.
Data: | 2006-11-01
Ano: | 2006
Palavras-chave: | Hypnea musciformis antifungal activity beta-1; 3-glucanase chitinase lectin.
Resumo: | A protein fraction, rich in lectin, obtained from the red seaweed Hypnea musciformis by precipitation with ammonium sulfate (F40/70) was screened for chitinase and beta-1,3-glucanase activity and assessed for antifungal potential against the human pathogen yeasts Candida albicans and C. guilliermondii. The F40/70 fraction showed chitinase and beta-1,3-glucanase enzymes, with specific activities of 276.43 and 1880.7 Units.mg -1 protein, respectively. It was capable of inhibiting the growth of C. guilliermondii at the concentrations of 45, 100 and 450 µg protein.ml -1 but it showed only a discrete inhibition against C. albicans irrespective of the tested concentrations. The inhibitory action was shown to be fungistatic and the presence of the glycoprotein fetuin, for which the lectin in the fraction had affinity, abolished the antifungal action. The complete growth recovery following fetuin treatment indicated that chitinase and beta-1,3-glucanase were not involved in the growth inhibition of these yeasts.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700008
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132006000700008
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.49 n.6 2006
Fonte: | info:eu-repo/semantics/openAccess