Data/hora: 11/06/2024 04:00:52

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar	
Autores: 	Montañez Sáenz,Julio; Téllez,Alejandro; de la Garza,Heliodoro; de la Luz Reyes,María; Contreras-Esquivel,Juan Carlos; Aguilar,Cristóbal Noé.	
Data: 	2000-01-01	
Ano: 	2000	
Palavras-chave: 	Extraction; Purification; Properties; Pectinesterase; Potato.	
Resumo: 	Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132000000400007	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.43 n.4 2000	
Fonte: 	info:eu-repo/semantics/openAccess