Data/hora: 17/06/2024 14:36:54

Provedor de dados: | 52
País: | Brazil
Título: | Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus
Autores: | Rezende,Sebastião Tavares de; Guimarães,Valéria Monteze; Rodrigues,Marília de Castro; Felix,Carlos Roberto.
Data: | 2005-03-01
Ano: | 2005
Palavras-chave: | Aspergillus fumigatus; Alpha-galactosidase; Raffinose oligosaccharides.
Resumo: | Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by gel filtration and two ion exchange chromatographies and showed Mw of 54.7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 °C, and retained about 80% of the original activity after incubation for 90 minutes at 50ºC. The KM for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200005
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132005000200005
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.48 n.2 2005
Fonte: | info:eu-repo/semantics/openAccess