Data/hora: 11/06/2024 14:47:54

Provedor de dados: | 52
País: | Brazil
Título: | Interference of a commercial catalase preparation in laccase and peroxidase activities
Autores: | Ballaminut,Nara; Yamanaka,Renata; Machado,Katia Maria Gomes.
Data: | 2009-10-01
Ano: | 2009
Palavras-chave: | Peroxidase; Fungi; Basidiomycetes; Hydrogen peroxide; Enzymatic inhibition.
Resumo: | The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500017
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000500017
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.5 2009
Fonte: | info:eu-repo/semantics/openAccess