Data/hora: 01/06/2024 06:16:46

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Multiple gene sequence analysis using genes of the bacterial DNA repair pathway	
Autores: 	Rotelok Neto,Miguel; Galvão,Carolina Weigert; Cruz,Leonardo Magalhães; Guizelini,Dieval; Silva,Leilane Caline; Garcia,Jarem Raul; Etto,Rafael Mazer.	
Data: 	2015-06-01	
Ano: 	2015	
Palavras-chave: 	Bacterial genome; Multiple gene sequence analysis; Taxonomic assignment; DNA repair genes; HGT.	
Resumo: 	The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways. Despite the amount of available genes in public database, the usual method to place genomes in a taxonomic context is based mainly on the 16S rRNA or housekeeping genes. Thus, the relationships among genomes remain poorly understood. In this work, an approach of multiple gene sequence analysis based on genes of DNA repair pathway was used to compare bacterial genomes. Housekeeping and DNA repair genes were searched in 872 completely sequenced bacterial genomes. Seven DNA repair and housekeeping genes from distinct metabolic pathways were selected, aligned, edited and concatenated head-to-tail to form a super-gene. Results showed that the multiple gene sequence analysis using DNA repair genes had better resolution at class level than the housekeeping genes. As housekeeping genes, the DNA repair genes were advantageous to separate bacterial groups at low taxonomic levels and also sensitive to genes derived from horizontal transfer.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300421	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-8913201500474	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.58 n.3 2015	
Fonte: 	info:eu-repo/semantics/openAccess