Data/hora: 03/06/2024 10:58:30

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil	
Autores: 	Nwagu,Tochukwu Nwamaka; Okolo,Bartholomew Ndubuisi.	
Data: 	2011-08-01	
Ano: 	2011	
Palavras-chave: 	Hydrolysis; Starch; Fungal growth; Amylase synthesis.	
Resumo: 	In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132011000400002	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.54 n.4 2011	
Fonte: 	info:eu-repo/semantics/openAccess