Data/hora: 06/06/2024 00:45:10

Provedor de dados: 	52	
País: 	Brazil	
Título: 	A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4	
Autores: 	Acevedo,Francisca; Pizzul,Leticia; Castillo,María del Pilar; Rubilar,Olga; Lienqueo,María Elena; Tortella,Gonzalo; Diez,María Cristina.	
Data: 	2011-12-01	
Ano: 	2011	
Palavras-chave: 	White-rot fungi; Ligninolytic enzymes; Purification; Lignocellulosic material.	
Resumo: 	In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132011000600013	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.54 n.6 2011	
Fonte: 	info:eu-repo/semantics/openAccess