Data/hora: 01/06/2024 05:48:30

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation	
Autores: 	Ribas,Alessandra Ferreira; Kobayashi,Adilson Kenji; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga Esteves.	
Data: 	2006-01-01	
Ano: 	2006	
Palavras-chave: 	Genetic transformation; Coffee; Herbicide tolerance; Ammonium glufosinate.	
Resumo: 	Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl)-adenine (2-iP). Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100002	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132006000100002	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.49 n.1 2006	
Fonte: 	info:eu-repo/semantics/openAccess