Data/hora: 16/06/2024 00:57:55

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Development of an enzyme immunoassay for poliovirus antigens	
Autores: 	Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko.	
Data: 	2007-01-01	
Ano: 	2007	
Palavras-chave: 	Enzyme immunoassay; Poliovirus antigen.	
Resumo: 	An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132007000100005	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.50 n.1 2007	
Fonte: 	info:eu-repo/semantics/openAccess